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KMID : 0385220010110020014
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Korean Journal of Gerontology
2001 Volume.11 No. 2 p.14 ~ p.20
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Investigation of Anti-aging Effect and Determination of Chemical Structure of Pine Needle (Pinus densiflora) through Animal Experiment Ⅳ. Effects of Ethyl Acetate Fraction on Oxygen Radicals and Their Scavenger Enzymes in Liver of SD Rats
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Choi Jin-Ho
Kim Dae-Ik
Kim Chang-Mok
Kim Hyun-Sook
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Abstract
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Male Spragure-Dawley rats were fed basic diets (control) and experimental diets added 25, 50 and 100 mg of EtOAc fraction fractionated from pine (Pinus dens flora Sieb et Zucc.) needle methanol extract for 45 days. Cholesterol accumulations in liver were dose- dependently inhibited (5.8%-8.1%, 1.7%-6.6%) in mitochondria and microsomes of these three EtOAc groups, but was significantly inhibited in mitochondria only of EtOAc-100 gruop compared with control group. Hydroxyl radical (.OH) formations were significantly inhibited (10.2% and 11.2%, 7.5% and 13.6%, respectively) in liver mitochondria and microsomes of EtOAc-50 and EtOAc-100 groups, and superoxide radical(O2) formations also were significantly inhibited (9.7% and 15.5%, respectively) in liver cytosol of EtOAc-50 and EtOAc-100 groups compared with control group. Cu/Zn-SOD activities were significantly increased (8.3%, 9.2% and 17.5%, respectively) in liver cytosol of these three EtOAc groups, but Mn-SOD activity was significantly increased about 10% in liver mitochondria of EtOAc-100 group compared with control group. GPx activities also were effectively increased (7.7%, 9.0%, 9.5%, respectively) in liver cytosol of these three EtOAc fraction, but GPx activities were significantly increased in liver cytosol of EtOAc-50 and EtOAc-100 groups only compared with control group. These results suggest that EtOAc fraction of pine needle may play an effective role in an attenuating oxygen radicals and activating scavenger enzymes, thus aging may be effectively modulated and inhibited.
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KEYWORD
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Pine(Pinus dens flora Sieb et Zucc.), oxygen radical, scavenger enzymes, superoxide dismutase(SOD), glutathione peroxidase(GPx)
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